Perbedaan Sekuens Asam Amino Epitop Sel B dan Sel T pada Protein Hemaglutinin (H) Antara Virus Campak Liar dan Virus Vaksin di Indonesia

Made Setiawan, Agus Sjahrurachman, Fera Ibrahim, Agus Suwandono

Sari


Latar belakang. Protein H virus campak sangat penting agar virus dapat menginfeksi sel pejamu. Selain itu, protein H dapat merangsang antibodi spesifik yang dapat menetralisasi virus campak, sehingga virus tidak dapat menginfeksi sel. Bila ada perbedaan sekuens asam amino epitop sel B dan sel T pada protein H antara virus campak liar dan virus vaksin campak, maka vaksin tidak dapat merangsang terbentuknya antibodi protektif.
Tujuan. Mengetahui perbedaan sekuens asam amino epitop sel B dan sel T pada protein H antara virus campak liar (G2, G3, dan D9) dan virus vaksin CAM-70, Schwarz dan Edmonston-wt.
Metode. Ekstraksi dan amplifikasi gen dilakukan di laboratorium menggunakan teknologi biologi molekuler dan analisis gen dan protein dilakukan menggunakan teknologi bioinformatika.
Hasil. Ditemukan perbedaan sekuens asam amino epitop sel T pada protein H antara virus campak liar dan virus Edmonstone-wt, sedangkan antara virus campak liar dan virus vaksin (CAM-70 dan vaksin Schwarz) tidak ditemukan perbedaan. Ditemukan perbedaan sekuens asam amino pada epitop sel B protein H antara virus campak liar dan virus vaksin (CAM-70 dan Schwarz, sedangkan antara CAM-70 dan Schwarz tidak ditemukan perbedaan.
Kesimpulan. Tidak ada perbedaan sekuens asam amino epitop sel T antara virus campak liar dan virus vaksin (Schwarz dan CAM-70). Perbedaan ditemukan pada epitop sel B antara virus campak liar dan virus vaksin (CAM-70 dan Schwarz).


Kata Kunci


virus campak; protein hemaglutinin; epitop sel T; epitop sel B

Teks Lengkap:

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Referensi


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DOI: http://dx.doi.org/10.14238/sp10.3.2008.190-5

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