Nilai Diagnostik Neutrophil Gelatinase-Associated Lipocalin Urin, Kelainan Urinalisis, dan Kombinasi Keduanya pada Infeksi Saluran Kemih Anak Usia 2–5 Tahun

Citra Estetika, Sudung O. Pardede, Zakiudin Munasir, Bambang Supriyatno, Titis Prawitasari, Piprim Basarah Yanuarso

Sari


Latar belakang. Infeksi saluran kemih (ISK) pada anak memiliki manifestasi klinis yang tidak khas sehingga sulit untuk diagnosis dini. Biakan urin memerlukan waktu hingga lima hari sehingga dapat menyebabkan keterlambatan terapi serta tingginya komplikasi ISK. Kelainan urinalisis yang saat ini digunakan masih memiliki spesifisitas yang rendah.
Tujuan. Mengetahui nilai diagnostik Neutrophil gelatinase-associated lipocalin (NGAL) urin, kelainan urinalisis, dan kombinasi keduanya untuk mendiagnosis dini ISK pada anak usia 2-5 tahun.
Metode. Uji diagnostik menggunakan biakan urin sebagai baku emas dengan desain potong lintang pada anak berusia 2-5 tahun dengan tersangka ISK dan dirawat di Rumah Sakit Dr. Cipto Mangunkusumo.
Hasil. Pemeriksaan NGAL urin diketahui memiliki sensitivitas 85,7% (IK95%: 63,6-96,9%), spesifisitas 74,3% (IK95%: 57,8-86,9%), positive predictive value 64,3% (IK95%: 50,6–75,9%), dan negative predictive value 90,6% (IK95%: 76,9-96,5%) pada anak dengan minimal satu kelainan urinalisis. Pemeriksaan NGAL urin hanya meningkatkan spesifisitas kelainan urinalisis berupa leukosituria saja dan tidak meningkatkan spesifisitas pada yang telah memiliki tiga kelainan urinalisis.
Kesimpulan. Neutrophil gelatinase-associated lipocalin urin tidak dianjurkan untuk meningkatkan spesifisitas urinalisis dalam diagnosis ISK pada anak usia 2–5 tahun. Gabungan tiga kelainan urinalisis tanpa NGAL urin sudah memiliki spesifisitas yang baik. Perlu dilakukan penelitian biomarker lain untuk mendiagnosis dini ISK dengan lebih baik.


Kata Kunci


neutrophil; gelatinase-associated; lipocalin; urinalisis; infeksi

Teks Lengkap:

PDF

Referensi


Yilmaz A, Sevketoglu E, Gedikbasi A, dkk. Early prediction of urinary tract infection with urinary neutrophil gelatinase associated lipocalin. Pediatr Nephrol 2009;24:2387-92.

Kim BH, Yu N, Kim HR, dkk. Evaluation of the optimal neutrophil gelatinase-associated lipocalin value as a screening biomarker for urinary tract infections in children. Ann Lab Med 2014;34:354-9.

Saadi S, Tabatabaei SM, Hooshmandi Z, dkk. Neutrophil gelatinase-associated lipocalin value in patients’ urine with gram positive cocci infection in the urinary tract. Int J Med Lab 2019;6:288-95.

Pardede SO. Infeksi pada ginjal dan saluran kemih anak: manifestasi klinis dan tata laksana. Sari Pediatri 2018;19:364-74.

Shalabi MS, Safdar OY, Shalaby MA, dkk. Urinary neutrophil gelatinase associated lipocalin for early UTI diagnosis. J Med Diagn Meth 2016;5:6-7.

Mattoo TK, Shaikh N, Nelson CP. Contemporary management of urinary tract infection in children. Pediatrics 2021;147:1-12.

Mori R, Yonemoto N, Fitzgerald A, dkk. Diagnostic performance of urine dipstick testing in children with suspected UTI: a systematic review of relationship with age and comparison with microscopy. Acta Paediatr 2010;99:581-4.

Robinson JL, Finlay JC, Lang ME, dkk. Urinary tract infection in infants and children: diagnosis and management. Pediatr Child Health 2014;6:315-9.

Utsch B, Klaus G. Urinalysis in children and adolescents. Dtsch Arztebl Int 2014;111:617-26.

Safdar OY, Shalaby M, Toffaha WM, dkk. Neutrophil gelatinase-associated lipocalin as an early marker for the diagnosis of urinary tract infections in Saudi children. J Nephrol Ther 2015;5:2-6.

Lubell TR, Barasch J, Xu K, dkk. Urinary neutrophil gelatinase–associated lipocalin for the diagnosis of urinary tract infections. Pediatrics 2017;140:1-10.

Jagadesan I, Agarwal I, Chaturvedi S, dkk. Urinary neutrophil gelatinase associated lipocalin: a sensitive marker for urinary tract infection in children. Indian J Nephrol 2019;29:340-4.

Leung AKC, Wong AHC, Leung AAM, dkk. Urinary tract infection in children. Recent Pat Inflamm Allergy Drug Discov 2019;13:2-18.

Miesien M, Tambunan T, Munasir Z. Profil klinis infeksi saluran kemih pada anak di RS Dr. Cipto Mangunkusumo. Sari Pediatri 2006;7:200-6.

Karita D, Romdhoni MF. Hubungan usia dan berat badan dengan ukuran lingkar penis anak menggunakan o-meter: sirkumsisi metode klem. Herb Med J 2018;1:1-7.

Mod HK, Jeeyani HN, Shah BM. Urinary tract infection in children: clinical aspects and utility of urine dipstick test. Int J Contemp Pediatrics 2017;4:790-5.

Daniel M, Grzelak HS, Sierdzinski J, dkk. Epidemiology and risk factors of UTIs in children: a single-center observation. J Pers Med 2023;13:1-13.

Atay N, Gokceoglu AU. Evaluation of urinalysis and urine culture in children with first-time urinary tract infection. Turk J Urol 2021;47:242-7.

Korbel L, Howell M, Spencer JD. The clinical diagnosis and management of urinary tract infections in children and adolescents. Pediatr Int Child Health 2017;37:273-9.

Terlizzi ME, Gribaudo G, Maffei ME. Uropathogenic Eschericia coli (UPEC) infections: virulence factors, bladder responses, antibiotic, and non-antibiotic antimicrobial strategies. Front Microbiol 2017;8:1-23.

Abraham SN, Miao Y. The nature of immune responses to urinary tract infections. Nat Rev Immunol 2015;15:655–63.

Hansen MA, King MV, Zoorob R, dkk. Prevalence and predictors of urine culture contamination in primary care: a cross-sectional study. Int J Nurs Stud 2022;134:1-22.

Khalifa MA, Abdoh AA, Silva FG, dkk. Interpretation of multiple isolate urine cultures in adult male patients. J Natl Med Assoc 1995;87:141-7.

Millner R, Becknell B. Urinary tract infections. Pediatr Clin N Am 2019;66:1-13.

Pardede SO, Tambunan T, Alatas H, dkk. Konsensus infeksi saluran kemih pada anak. Jakarta: Badan Penerbit Ikatan Dokter Anak Indonesia; 2011.

Fernandes DJ, Jaidev MD, Castelino DN. Utility of dipstick test (nitrite and leukocyte esterase) and microscopic analysis of urine when compared to culture in the diagnosis of urinary tract infection in children. Int J Contemp Pediatr 2018;5:156-60.

Hirmas N, Mubarak S, Sultan I. Patterns of microbial growth in urine cultures in a pediatric hematology/oncology unit over a one-year period: a single institution study. Int J Pediatr Adolesc Med 2017;4:95-9.

Zgheib H, Zakhem AE, Wakil C, dkk. Role of urine studies in asymptomatic febrile neutropenic patients presenting to the emergency department. World J Emerg Med 2021;12:99-104.

Rangaiahagari A, Nyirabanzi J, Uwizeyimana JP, dkk. Comparison of urine culture and urine dipstick nitrite test in urinary tract infection. Rwanda Med J 2015;1:1-7.

Huysal K, Budak YU, Karaca AU, dkk. Diagnostic accuracy of urised automated urine microscopic sediment analyzer and dipstick parameters in predicting urine culture test results. Biochem Medica 2013;23:211-7.

Bacarea A, Fekete GL, Grigorescu BL, dkk. Discrepancy in results between dipstick urinalysis and urine sediment microscopy. Exp Ther Med 2021;21:538-90.

Daghistani HI, Dayem AM. Diagnostic value of various urine tests in the Jordanian population with urinary tract infection. Clin Chem Lab Medic 2002;40:1048-51.

Watts S, Bryan D, Marill K. Is there a link between hyperbilirubinemia and elevated urine nitrite. Am J Emerg Med 2007;25:10-4.

Khairina A, Trihono PP, Munasir Z. Urinalysis as a diagnostic tool for febrile urinary tract infection in children aged 2 months–2 years. Psediatr Indones 2014;54:101-8.

Devarajan P. Neutrophil gelatinase-associated lipocalin (NGAL): a new marker of kidney disease. Scand J Clin Lab Invest 2008;68:89-94.

Ning M, Mao X, Niu Y, Tang B, dkk. Usefulness and limitations of neutrophil gelatinase-associated lipocalin in the assessment of kidney diseases. J Lab Precis Med 2018;3:1-10.

Bladzinska AG, Zylka A, Dumnicka P, dkk. Sterile leukocyturia affects urine neutrophil gelatinase-associated lipocalin concentration in type 2 diabetic patients. Arch Med Sci 2017;13:321-7.

Decavele ASC, Dhondt L, Buyzere ML, dkk. Increased urinary neutrophil gelatinase associated lipocalin in urinary tract infections and leukocyturia. Clin Chem Lab Med 2011;49:999-1003.

Forster CS, Jackson E, Ma Q, dkk. Predictive ability of NGAL in identifying urinary tract infection in children with neurogenic bladders. Pediatr Nephrol 2018;33:1365-74.

Urbschat A, Obermüller N, Paulus P, dkk. Upper and lower urinary tract infections can be detected early but not be discriminated by urinary NGAL in adults. Int Urol Nephrol 2014;46:2243-9.




DOI: http://dx.doi.org/10.14238/sp26.3.2024.137-45

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

##submission.license.cc.by-nc-sa4.footer##

Informasi Editorial:
Badan Penerbit Ikatan Dokter Anak Indonesia
Jl. Salemba I No 5, Jakarta 10430, Indonesia
Phone/Fax: +62-21-3912577
Email: editorial [at] saripediatri.org

Lisensi Creative Commons
Sari Pediatri diterbitkan oleh Badan Penerbit Ikatan Dokter Anak Indonesia
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi-NonKomersial-BerbagiSerupa 4.0 Internasional.